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Journal: Journal of Cardiovascular Development and Disease
Article Title: Vitamin D Inhibits IL-6 Pro-Atherothrombotic Effects in Human Endothelial Cells: A Potential Mechanism for Protection against COVID-19 Infection?
doi: 10.3390/jcdd9010027
Figure Lengend Snippet: ( A ) In HUVEC incubated with IL-6, a significant increase of NF-kB levels was observed. Pretreatment of endothelial cells with VitD prevented the IL-6 effects on NF-kB. LPS served as positive control. Each bar represents the mean ± SD of 3 different experiments. (* p < 0.001 vs. Control; ** p < 0.001 vs. IL-6, one-way ANOVA with Tukey’s post hoc test). ( B ) Western blot analyses. IL-6 stimulation results in significant reduced levels of IκB. Conversely, preincubation with VitD restores IκB levels. LPS served as positive control. Each bar represents the mean ± SD of 3 different experiments. (* p < 0.001 vs. Control; ** p < 0.001 vs. IL-6, one-way ANOVA with Tukey’s post hoc test). The insert shows results of a representative experiment. ( C ) In HUVEC incubated with IL-6, a significant increase of STAT3 levels was observed. Pretreatment of endothelial cells with VitD prevented the IL-6 effects on STAT3. LPS served as positive control. Each bar represents the mean ± SD of 3 different experiments. (* p < 0.001 vs. Control; ** p < 0.001 vs. IL-6, one-way ANOVA with Tukey’s post hoc test).
Article Snippet:
Techniques: Incubation, Positive Control, Control, Western Blot